View and modify DNA constructs, share the files with your coworkers around the globe. Pick DNA fragments and have gene fusions automatically created, insert pieces into a plasmid without the need for restriction enzymes, simulate a cloning mechanism with an error detected and corrected.
Genetic research and biochemistry are very important today since they can help humans to fight infectious and hereditary diseases and create new and enhanced species.
SnapGene is a program that allows users to view, edit, and annotate biochemical sequences, such as proteins and DNA. It has a colorful interface that makes these tasks more user-friendly. It also makes extensive use of graphics, so the information is displayed in a clear manner.
The program has a huge amount of features that allow you to simulate the cloning of DNA sequences, the insertion of DNA fragments into a sequence, align DNA to a chromosome, and more.
Please note that the program is aimed to scientists and specialized professionals, and not to the casual or novice user. Thus, you need a solid previous knowledge to use this program and take advantage of all its features.
You can try the program before buying a license. To do so, you need to enter a valid e-mail address and click on the link you will receive. You can use the program during one full month. If you find the program useful, you can purchase a yearly or permanent license whose cost will vary, depending on whether you use it for academic or for-profit purposes, as well as on the amount of users.
v2.5 [Sep 15, 2014]
Functionality
- Gateway Cloning
- You can now insert or ligate up to eight restriction fragments.
- You can now overlap up to eight fragments when performing Overlap Extension PCR.
- InFusion® Cloning now supports up to 8 fragments.
- Print or export an inverted agarose gel.
- Import a Range of Records from GenBank.
- Import a region of a sequence from GenBank.
- Import one or more primers copied to the clipboard.
- Improved importing from Vector NTI Databases and ma4 archives to include sequence history, creation and modification dates, the sequence author, user comments, as well as maintain the creation / modification date and time in the resulting .dna files that are produced.
- Added Thermo Scientific's "GeneRuler™ High Range DNA Ladder"
- Added KAPA Biosystems' Express and Universal ladders.
- Added Fisher Scientific Ladders.
- Added SERVA DNA Ladders.
- Added Thermo Scientific's MassRuler ladders.
Enhancements
- Reorganized the Choose Restriction Enzymes dialog to make it easier to find/use the controls for automatically select enzymes based on a criteria.
- The number of binding sites are now shown in primer tooltips.
- Increased the maximum number of recent documents listed to 25.
- Enhanced the GenBank importer to decode base numbering if specified in a REGION indicator within the ACCESSION section.
- SnapGene can now open malformed GenBank files produced by Vector NTI Express.
- Added a yellow warning to the Add/Edit/Duplicate Primer dialogs when a primer has multiple binding sites.
- Enhanced the look and feel of directionality buttons when a window loses focus.
- Now decoding /Design_Description qualifiers in VNTI files as /note qualifiers.
- Improved the mouse cursor while over or dragging a splitter.
- Improved Actions menu layout.
- Various optimizations, textual and tooltip enhancements.
Bug Fixes
- Fixed a bug where selected non-cutters were not listed when printing Agarose Gels.
- Fixed a bug where feature names could overlap each other in circular maps.
- Fixed various bugs with creating features from an enzyme selection whose right end is the numerical origin.
- Fixed a bug where full-sequence "source" type features were changed to "misc_feature" when exported using the Vector NTI GenBank format.
- Fixed a bug with copying while using the Edit and Duplicate Primer dialogs.
- Fixed a bug that could result in bogus primers being shown for the current sequence in History View and a crash that could occur while interacting with these bogus elements.
- Fixed a potential hang that could occur while importing VectorNTI databases.
- Fixed a bug where after editing the list of MW markers, the pull down menu did not resize.
- When closing a window, the next visible window is now automatically active.
- Fixed a bug where when saved enzymes sets were modified, these changes were not shown in an already open PCR, Overlap Extension PCR, Mutagenesis, InFusion Cloning, or Gibson Assembly dialog.
- Fixed a weird glitch where buttons in the launch dialog in addition to others would appear to disappear when the window lost focus.
- Fixed a bug that prevented the Cmd [ and Cmd [ shortcuts from activating the Back/Forward buttons in the Restriction Enzymes dialog.
- Fixed a bug where copying from the primer sequence controls copied invisible formatting to the clipboard.
- SnapGene now refuses to open GenBank Protein sequences, informing the user that they are not yet supported at this time.
- Fixed a regression with drawing rounded ovals used for example for mouse position indicators.
- If a custom map label is used in Map View, we now use that custom map label for the root node in History View as well.
- Fixed a crash that could occur when performing range limited a highly degenerate MICA search.
- Fixed a bug that prevented alignment directionality symbols from showing up properly when printed to PDF on 10.9.
- Fixed various glitches with printing the aligned sequences summary when printing Sequence View.
- Fixed a bug that could result in aligned sequences reporting incorrect dates.
- Fixed various bugs with pressing and releasing Opt/Alt and updating menu actions.
- Fixed a regression that prevented "Go To" from working while viewing a sequence trace.
- Fixed various bugs with printing zoomed alignments.
- Fixed a bug that prevented "Replace Original with Aligned Sequences" from proceeding when using two more compatible aligned sequences if some but not all align around the numerical origin.
- Fixed a bug that could cause a list selection to change when activating a window on Mac OS X.
- Fixed a bug where when selecting an externally placed feature name in map view, the wrong DNA range was selected.
- Fixed various bugs with viewing origin-spanning alignments as double stranded sequences.
- Fixed a crash that could occur while using Sequence View.
- Fixed a crash that could occur while simulating agarose gels.
